AutoGenFlex STAR
An AutoGen and QIAGEN Collaboration

Using their expertise in nucleic acid isolation techniques and instrumentation, QIAGEN and AutoGen, Inc. have developed a new automated platform for isolating genomic DNA from large volumes of whole blood.  The AutoGenFlex combines the proven DNA isolation technology from QIAGEN and the robust and reliable instrumentation from AutoGen.  The AutoGenFlex is completely automated and easy to use, with no manual intervention required.  Isolate up to 80 x 5.0ml whole blood samples per day and produce consistently high quality and high yield DNA, ready for your downstream application.  In addition to whole blood, the AutoGenFlex STAR can isolate gDNA from tissues, cultured cells and Oragene collected saliva samples. 

The AutoGenFlex STAR is now available with an optional Sample Tracking And Reporting package.

Download AutoGenFlex 3000 Brochure (pdf)
Download NEW AutoGenFlex STAR Flyer (pdf)
Download AutoGenFlex Fresh Blood Protocol Sheet (pdf)
Download AutoGenFlex Frozen Blood Protocol Sheet (pdf)
More on the FlexiGene DNA AGF3000 Kit

Genomic DNA Tissue Protocol also available on AutoGenFlex using AutoGen Chemistry

NEW! Protocol for isolating gDNA from Oragene Collected Saliva Samples available on AutoGenFlex using AutoGen Chemistry

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The AutoGenFlex STAR provides:

        • Significant labor reduction - Completely automated walk-away system

        • Improved accuracy and reliability - Dependable DNA isolation with trouble-free robotic operation

        • Increased productivity - Frees lab personnel for other tasks

        • Compact size - Provides more space in your lab

        • Sample tracking by bar code identification (optional feature)

        • PC workstation for data management  (optional feature)
 

	Major components of the AutoGenFlex system include:
	Built-in Centrifuge		Reagent Dispensing
		• 4-position swing   type buckets • Variable speed   and duration settings • HEPA-Filter for   biohazard reduction		• 7 independent   motor driven   syringes • Addition of   multiple reagents • 2 containers for   waste separation
	Workstation	• Removable sample   and DNA storage   racks • Heater to allow for   efficient digesting of   the sample and   drying of purified DNA	Robotics	• High precision   gripper-type robot   hand • Transports   sample tubes to   and from      workstation,   dispensing station   and centrifuge
	Touch Panel Screen	• Touch panel for easy    access • Easy to use • Allows for parameter   changes • Stores customized   files	Sample Handling	• Two removable   trays for sample   handling • Sample input tray • DNA output tray

	The AutoGenFlex STAR provides an optional Sample Tracking And Reporting Package capability:
	Sample & Error Tracking Software	Sample Tracking Report
	Communication Port for Interface to PC Collects Bar Coded Sample Identification Data	Tracks Sample ID through sample input to DNA recovery and monitors Error Status of each run Prepares and stores Tracking Report for each run
	Sample Identification System
	Scan barcode on primary sample tube Scan barcode on sample input tray and tube units Scan barcode on DNA output tray and tube units Load patient samples from primary tube into tube units of sample input tray

The FlexiGene Chemistry on the AutoGenFlex provides:

        • High quality, high molecular weight DNA

        • More consistent removal of contaminants and inhibitors

        • Compatibility with all downstream applications

In contrast to standard salting out techniques FlexiGene DNA chemistry from QIAGEN begins with pelleting and collection of nuclei and mitochnodria, which results in more complete removal of RNA and other contaminants.  Subsequent protease digestion facilitates complete protein removal resulting in high quality and high molecular weight DNA.

The purified DNA performs well in a range of downstream applications including PCR-based techniques, restriction digestion, Southern blotting, and sequencing. 

Example of Genomic DNA obtained from whole blood:

Genomic DNA was isolated on the AutoGenFlex using the FlexiGene 5ml whole blood protocol.  The starting material was 5.0 ml of EDTA treated whole blood from four different donors with five replicates each.  The yield from 5.0 ml whole blood is 100-210 μg genomic DNA with a high molecular weight of 30–150 kb.