Automated Nucleic Acid Extraction in a Bench Top Package!

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The high quality of purified DNA samples is critical to successful genotyping studies. DNA samples of high quality and low cleavage rates are particularly essential in studies involving many genetic markers. QuickGene-610L isolates DNA samples under gentle extraction conditions, without using conventional centrifugal processes. This results in higher yields of DNA with higher molecular weight, fewer cleavages and high purity. QuickGene-610L is able to extract genomic DNA from a 2ml whole blood sample, and able to process 6 samples within 12 minutes.(*)     * Over 2ml of whole blood sample is adapted to pellet cells (buffy coat sample). Only three steps are required after preparing lysate samples: apply sample to cartridge, place cartridge holder on instrument and press START. QuickGene-610L's pre-programmed protocol automates the rest of the process. Flat-paneled design and detachable stands allow easier maintenance and cleaning. While running QuickGene-610L, another 6 samples can be applied to another Carriage holder. The system can process continuously by switching the cartridge holder. QuickGene-610L can perform 60 sample extractions per hour. It really is a high throughput machine! FUJIFILM’s thin membrane effectively absorbs nucleic acids in hydrophobic solvents, separating proteins and lipids. This results in high purity and a greatly reduced processing time compared to manual methods. DNA samples isolated by QuickGene­610L require no further purification. Extracted DNA can be directly applied to genetic analyses studies, including PCR and SNP validation. It simplifies the routine analytical workflow, and results in high throughput processing. The purification process removes proteins and salts producing high quality DNA that can be used directly in most downstream applications. About 50µ g of genome DNA can be extracted from 2ml of whole blood. The low pressure, gentle separation process employed by the QuickGene produces long DNA molecules up to 97 kbp.  Traditional spin-column extraction methods with a centrifuge often yield shorter DNA fragments than QuickGene due to the frequent cleavage of DNA molecules. The extracted DNA can be applied directly to PCR analysis. It is possible to perform PCR amplification with only a small volume of the high quality DNA produced by the QuickGene.