{AutoGenprep 245T}

I.       Purpose:    To extract genomic DNA from animal tissue.

II.    Chemical Principal:  Modified CTAB/Proteinase K digestion & Organic extraction method.

III. Protocol Parameters:

A.    Sample Volume^*1:  5-10mg (Mouse liver, lung, brain and uterus)
                                10-15mg (Mouse tail, heart and testis)
                                ^*1 Optimal starting volume depends on type of animal and tissue.

B.    Maximum Number of Samples: 24 samples per run.

C.    Processing Time: 1.8 hours for 12 samples^*2
                              2.5 hours for 24 samples^{*2
                              *2} This includes drying and resuspending DNA steps.

D.  Yield^*3:  3~6 μg of gDNA / 1mg of liver
             0.8~2 μg of gDNA / 1mg of other types of tissues.
             ^*3 Measured by OD260 value.  Actual yield may vary, depending on the condition and type of tissue sample.

E.     Quality:    Typical OD_260/280 values in water are ³ 1.70 - 1.95
             The DNA can be used directly in downstream processes such as
                PCR, real time PCR and restriction endonuclease digestions.

IV.     Running the Protocol:

       A.    Load Reagents and Samples
         B.    Select a Protocol
         C.    Enter Number of Samples
         D.     Start the Run

V. Example of genomic DNA:

          A. Extracted genomic DNA:

               Gel Electrophoresis:
               Sample: Approx. 0.5-3.0 ug of genomic DNA was loaded onto the gel
               Gel: 0.7% agarose gel, 1xTBE, 50V x 1.5hr

                                           Mouse tail

                Mouse Liver

                Mouse Lung

             B. Typical Yield and Purity⁺

VI.    Extraction and Purification Process: