{AutoGenprep 965, 960}

I.       Purpose:  To extract genomic DNA from plant material.

II.    Chemical Principal:  Modified CTAB/SDS/KOAc method.

III.  Pretreatment of Plant:

A.     50mg (wet) of plant material is frozen in liquid nitrogen and ground using a pestle or a
commercially available grinder.

B.     After Plant lysis solution (Reagent M1) is added, the ground sample is digested at 65°C for 30
minutes.

IV. Protocol Parameters:

A.    Sample Volume: 0.3 ml of digested solution.

B.    Maximum Number of Samples: 384 samples (4 plates) per run.

C.    Processing Time:     2.7 hours for 192 samples (2 plates)
                                  4.4 hours for 384 samples (4 plates)
                                  This includes a 20 minute drying period.

D.    Yield:     5.0 mg of purified genomic DNA from 50mg (wet) of young rice [Oryza sativa] leaf.
              Note: The actual yield may vary depending on a number of factors, including type,                        
                         part and maturity of plant material.

E.     Quality:     Typical OD_260/280 values are 1.75 - 1.90
                  The DNA can be used directly in downstream applications such as fluorescent DNA
                  sequencing, PCR and restriction endonuclease digestions.

V.  Running the Protocol:

        A.    Load Reagents, Tips and Samples
        B.    Select a Protocol
        C.    Enter Number of Samples
        D.     Start the Run

VI. Example of DNA extracted on the AutoGenprep 965/960:

   M   1      2     3     4      5     6     7      8     9   10    11     12

Gel electrophoresis of the extracted Plant DNA
(0.7% agarose gel in TAE, 50Vx1hr)

M: Hind III marker
Lanes 1-12: DNA extracted on the AutoGenprep 965/960

VII. Extraction Process: